Lipidomics Facility
Your partner for lipid analysis and fingerprinting
Lipidomics is the large-scale study of pathways and networks of cellular lipids in biological systems. The enormous structural diversity of lipids requires complex regulation at multiple spatial and temporal scales in biological systems and we are only beginning to understand the role of individual lipid species. Mass spectrometry MS-based lipidomic analysis is rapidly advancing this field. The Lipidomics Facility houses chromatographic and mass spectrometric equipment that is required for the analysis of complex lipid mixtures and performs data interpretation using bioinformatics.
Equipment
The available technologies include MS-based lipidomics techniques (three mass spectrometers, equipped with ion sources (turbo-)ESI, nano-ESI, and APCI, autosamplers, micropumps, UV detectors, UPLC and variable wavelength Fluorescence detector), assays for lipid metabolism and lipid flux using heavy isotope labeled precursors.
Analysis
High-throughput technologies have been developed in our laboratory allowing automated lipid analysis of 300 samples per day. We built a web-based ontology enrichment tool (LION/web) for lipidomic data analysis, allowing the handling of the large amounts of data and the translation of results to interpret the involvement of lipids in biological systems. This allows the understanding how lipids, together with proteins, determine cellular functions in health and disease.
Services
- Molecular analysis of a broad spectrum of (phospho)lipids (lipid fingerprinting)
- Biomarker identification
- Analysis and identification of unusual lipids.
- Lipid flux studies
- Semiquantitative and quantitative analysis
- Bioinformatic analysis and data interpretation
Contact
Bernd Helms
Lipidomics Facility
Yalelaan 2
3584 CL Utrecht
Phone: +31 30 253 5375
Email: j.b.helms@uu.nl
Website: www.uu.nl/en/organisation/faculty-of-veterinary-medicine/lipidomics-facility