Flow Cytrometry and Cell Sorting Facility
Flow cytometry
Flow cytometry literally means the measurement of cells in flow. In real life it means that any suspension of particles within the size range of approximately 50 micrometer to 100 nanometer can be measured. The measurements can be performed at high speed, on average up to 20,000 per second. In this way, data can be obtained from each individual particle and high numbers of particles can be measured in a short period of time. This technique is extremely suitable for measuring sub-populations (e.g. of cells) within a suspension. In addition, individual cells or particles can be purified using cell sorting.
Services
The facility has accumulated specific expertise on measurement and sorting of cells since 1998. This expertise builds on the experience accumulated with a wide variety of analyses and purifications, e.g. with immune cells, stem cells, sperm cells, chromosomes, bacteria, hybridoma's for monoclonal antibody production, single cell sorting for cloning and/or PCR analysis, cell selection based on fluorescent reporter gene products, rare event purifications and the high resolution analysis and sorting of sub-micron particles. We are trained to rethink seemingly impossible demands into successful experiments.
- For analysis of suspensions we can accommodate experiments requiring lasers with the following wavelength: 405 nm, 488 nm 561 nm, 640 nm and 806 nm. Up to 25 parameters can be measured simultaneously;
- For purification we can accommodate experiments requiring lasers with the following wavelength: Ultra Violet, 405 nm, 457 nm 488 nm, 561 nm and 640 nm;
- For sorting, up to 4 populations can be selected simultaneously in one run. There is a choice of collection modules like multi-well trays, tubes or slides where cells can be directly sorted in.
Contact
Dr. Estefan铆a Lozano Andr茅s
Centre for Flow Cytometry
Division of Infection & Immunology
Department of Biomolecular Health Sciences
Faculty of Veterinary Medicine
Utrecht 木瓜福利影视
Yalelaan 1
3584 CL Utrecht
Phone: +31 30 253 5833 or +31 30 253 5835
Email: e.lozanoandres@uu.nl
Website: www.uu.nl/en/organisation/faculty-of-veterinary-medicine/flow-cytometry-and-cell-sorting