Core Flow Cytometry

We think in solutions

The Core Flow Cytometry Facility is part of the Laboratory of Translational Immunology and provides state-of-the-art flow cytometry equipment, knowledge and high-speed cell sorting. The facility works with certification of ISO15189 and ISO9001, which makes it possible for research and diagnostics to work in close collaboration.

This UMC Utrecht facility cooperates with commercial and non-commercial partners, provides dedicated operators for assistance and is actively involved in flow cytometry education on different levels.

The facility is currently equipped with:

  • 6 FACS canto II, 3 lasers (405nm, 488nm, 635nm) - 2 LSRFortessa, 4 lasers (405nm, 488nm, 561nm, 635nm) 16 fluorescent parameters;
  • Aria II, 3 lasers (405nm, 488nm, 635nm) 10 fluorescent parameters and can sort and collect 4 different cell populations simultaneously;
  • Aria III, 4 lasers (405nm, 488nm, 561nm, 635nm) 16 fluorescent parameters and can sort and collect 4 different cell populations simultaneously;
  • 10 re-analysis PC鈥檚 with DIVA and Flowjo;
  • 6 channels Imagestream;
  • 6 way sorting, 70.000 ev/ps, 5 lasers, 35 parameters acquisition.

Cell sorting is done for:
Next gen sequencing, single cell cloning (6-384 wells plates), purifying genetic modified cells, isolation of (rare) cell populations and more.

Sorting and measuring conditions:

  • High speed and viability sorting;
  • Sorting/analysing at lab-, BSL 1-and BSL 2-level;
  • Index cell sorting;
  • High throughput analysis (96, 384 wells);
  • Up to 18 parameter cell analysis;
  • Minimal residual disease detection (1 of 1*106).

All analysers and high-speed cell sorters have uniformly standardised cytometer settings, for different sizes of particles, based on several years of diagnostic and research immunofenotypic knowledge. This standardisation protocol increases the interchangeability and makes it easily possible to participate in multi-center trails. 

Contact

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