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The Faculty of Science Flow Cytometry Facility houses three different machines that support a broad range of applications:
 

1. BD FACSymphony A1: equipped with a small particle detector for measuring particles as small as 90nm, and can be used for multiple-parameter analysis
    
2. BD FACSAria Fusion: used for both analysis and to rapidly sort and collect the particles of interest.
    
3.  Union Biometrica Biosorter: to process larger particles such as plant seeds or mammalian organoids, as well as whole organisms such as zebrafish embryos and C. elegans larvae.

For more information or to make use of one of the machines, please contact Toni van Capel (t.m.m.vancapel@uu.nl)

BD FACSymphony A1: cell analyzer with small particle detector

The FACSymphony A1 is used for analyzing particles, ranging from very small ones such as extracellular vesicles, to microorganisms such as yeasts, and a wide variety of blood and tissue cells. This analyzer supports high-resolution analysis of up to 16 parameters simultaneously. Examples of possible applications are measuring cell proliferation, apoptosis, transfection efficiency, cell cycle progression, cytometry bead array, and identification of rare subsets by multicolor staining (both intra- and extracellular).

Features

• 4 laser system (405, 488, 561 and 637 nm)
• Measures FSC, SSC + 14 fluorescent parameters
• Small particle detector
• Size range: ~90nm -30µm
• High-throughput sampler to automate sampling directly from 96- and 384-well plates

Overview lasers, filters and some examples of fluorochromes per detector:

BD FACSAria Fusion: cell sorter

The Aria Fusion is used for sorting particles, ranging from microorganisms such as yeasts to various blood or tissue cells. This is performed at high speed and in sterile conditions. Thereby facilitating the option to bring these cells back into culture, allowing creation of specific cell clones or performance of functional assays with the cells of interest. This sorter supports high-resolution analysis of up to 17 fluorescent markers simultaneously.

Features

• Located in an ML-II safety cabinet
• 5 laser system (355, 405, 488, 561 and 637 nm)
• Measures FSC, SSC + 17 fluorescent parameters
• Size range: ~200nm - 30µm
• Possibility to discriminate between GFP and YFP
• 1-4 Way sorting
• Sorting in Eppendorf tubes / 4 ml tubes / 15 ml tubes / 6, 12, 24, 96, 384-well plates / index sorting / Petri dishes
• Nozzles: 70, 85, 100, 130 µm
• Possibility to cool the sample and collection device
• Depending on nozzle size and pressure, sorting up to 5.000-22.000 cells/second

Overview lasers, filters and some examples of fluorochromes per detector:

Union Biometrica: Large Particle Biosorter

Some objects are too large or too sensitive for conventional flow cytometry. Therefore, Union Biometrica has developed large particle flow cytometers for handling a wide range of object sizes (1 – 1500 µm). Examples of materials that can be analyzed and sorted with the Biosorter are plant protoplasts, seeds, organoids and whole organisms, including zebrafish embryos and C. elegans embryos and larvae.

Relative size and optical density are measured, as well as fluorescence intensities at three different wavelengths. Sorting and dispensing decisions are based on user-selected ranges using the FlowPilotâ„¢ Pro software. Of each object, an optical profile can be made, in which the location and intensity of all parameters can be graphically mapped. This feature makes it possible to distinguish between fluorescence in the head, middle, or tail of the organism.

Features

• 3 laser system (405, 488 and 561 nm)
• Measures size, optical density + 3 fluorescent parameters
• Size range: ~1µm - 1500µm
• Partial profiling
• Particles ranging from 10-1500 µm
• 1 way sorting in Eppendorf tubes / 4ml tubes / 15ml tubes / 6, 12, 24, 96, 384-well plates / index sorting / Petri dishes
• Gentle sorting mechanism
• LP sampler for direct analysis from 96-well plates