Techniques used
In our group we use an array of molecular-, genetic-, bioinformatic- and biochemistry tools to understand plant functioning and response to environmental signalling. Techniques include, but are not limited to:
- General recombinant DNA technology. (assembly of DNA constructs, PCR, automated DNA sequence analysis; use of DNA analysis software; internet-based DNA databases analysis etc.).
- Genetic experiments with plants: crossings, genomic DNA isolations, Southern blotting, and molecular marker analysis such as CAPS, SNP and SSLP.
- Heterologous expression of genes in E.coli, Saccharomyces and Pichia and Yeast two-hybrid screens.
- Transient expression assays in plant protoplasts.
- Genetic modification of plants -mostly Arabidopsis- using the Agrobacterium tumefaciens mediated floral dip method. Selection of transgenic plantlines.
- Analysis of gene expression in wildtype, mutant and transgenic plants: Quantitative PCR (Q-PCR), and Western blotting techniques
- Genome wide expression profiling using Next Generation sequencing and DNA micro-arrays. (Web based) Data processing and bioinformatics.
- Physiological and biochemical analysis, e.g. sugar metabolism, responses to phytohormones and light. Automated advanced HPLC techniques. Protein isolations binding- and activity assays.
- Metabolomics
- Advanced cytogenetics techniques to visualise chromatin and quantify compaction states, including Fluorescent in situ hybridisation (FISH) and immunolabeling.
- Advanced microscopy, a.o. fluorescence microscopy and confocal microscopy.
- Chromatin Immunoprecipitation (ChIP) and ChiP-sequencing techniques to identify DNA binding sites of proteins
- Chemical genetics. Library screening, compound confirmation and optimization, application